54 research outputs found
Recommended from our members
A problem of overlap
Here we propose that earlier-demonstrated details in the primate visual cortical map may account for an otherwise puzzling (and problematic) finding in the current human fMRI literature. Specifically, the well-known regions LO and MT(+) reportedly overlap in the human cortical visual map, when those two regions are localized using standard stimulus comparisons in conventional fMRI experiments. Here we describe evidence supporting the idea that the apparent functional overlap between LO and MT arises from a third area (the MT crescent: âMTcâ), which is well known to surround posterior MT based on earlier histological, neuroanatomical, and electrophysiological studies in nonhuman primates. If we assume that MTc also exists in human visual cortex, and that it has a location and functional properties intermediate to those in LO and MT, simplistic modeling confirmed that this arrangement could produce apparent overlap between localizers for LO and MT in conventional fMRI maps in human visual cortex
Increased Visual Stimulation Systematically Decreases Activity in Lateral Intermediate Cortex
Previous studies have attributed multiple diverse roles to the posterior superior temporal cortex (STC), both visually driven and cognitive, including part of the default mode network (DMN). Here, we demonstrate a unifying property across this multimodal region. Specifically, the lateral intermediate (LIM) portion of STC showed an unexpected feature: a progressively decreasing fMRI response to increases in visual stimulus size (or number). Such responses are reversed in sign, relative to well-known responses in classic occipital temporal visual cortex. In LIM, this âreversedâ size function was present across multiple object categories and retinotopic eccentricities. Moreover, we found a significant interaction between the LIM size function and the distribution of subjects' attention. These findings suggest that LIM serves as a part of the DMN. Further analysis of functional connectivity, plus a meta-analysis of previous fMRI results, suggests that LIM is a heterogeneous area including different subdivisions. Surprisingly, analogous fMRI tests in macaque monkeys did not reveal a clear homolog of LIM. This interspecies discrepancy supports the idea that self-referential thinking and theory of mind are more prominent in humans, compared with monkeys
Recommended from our members
A parametric study of fear generalization to faces and non-face objects: relationship to discrimination thresholds
Fear generalization is the production of fear responses to a stimulus that is similarâbut not identicalâto a threatening stimulus. Although prior studies have found that fear generalization magnitudes are qualitatively related to the degree of perceptual similarity to the threatening stimulus, the precise relationship between these two functions has not been measured systematically. Also, it remains unknown whether fear generalization mechanisms differ for social and non-social information. To examine these questions, we measured perceptual discrimination and fear generalization in the same subjects, using images of human faces and non-face control stimuli (âblobsâ) that were perceptually matched to the faces. First, each subjectâs ability to discriminate between pairs of faces or blobs was measured. Each subject then underwent a Pavlovian fear conditioning procedure, in which each of the paired conditioned stimuli (CS) were either followed (CS+) or not followed (CSâ) by a shock. Skin conductance responses (SCRs) were also measured. Subjects were then presented with the CS+, CSâ and five levels of a CS+-to-CSâ morph continuum between the paired stimuli, which were identified based on individual discrimination thresholds. Finally, subjects rated the likelihood that each stimulus had been followed by a shock. Subjects showed both autonomic (SCR-based) and conscious (ratings-based) fear responses to morphs that they could not discriminate from the CS+ (generalization). For both faces and non-face objects, fear generalization was not found above discrimination thresholds. However, subjects exhibited greater fear generalization in the shock likelihood ratings compared to the SCRs, particularly for faces. These findings reveal that autonomic threat detection mechanisms in humans are highly sensitive to small perceptual differences between stimuli. Also, the conscious evaluation of threat shows broader generalization than autonomic responses, biased towards labeling a stimulus as threatening
Ultra-high spatial resolution BOLD fMRI in humans using combined segmented-accelerated VFA-FLEET with a recursive RF pulse design
Purpose
To alleviate the spatial encoding limitations of single-shot EPI by
developing multi-shot segmented EPI for ultra-high-resolution fMRI with reduced
ghosting artifacts from subject motion and respiration.
Methods
Segmented EPI can reduce readout duration and reduce acceleration factors,
however, the time elapsed between segment acquisitions (on the order of
seconds) can result in intermittent ghosting, limiting its use for fMRI. Here,
"FLEET" segment ordering--where segments are looped over before slices--was
combined with a variable flip angle progression (VFA-FLEET) to improve
inter-segment fidelity and maximize signal for fMRI. Scaling a sinc pulse's
flip angle for each segment (VFA-FLEET-Sinc) produced inconsistent slice
profiles and ghosting, therefore, a recursive Shinnar-Le Roux (SLR) RF pulse
design was developed (VFA-FLEET-SLR) to generate unique pulses for every
segment that together produce consistent slice profiles and signals.
Results
The temporal stability of VFA-FLEET-SLR was compared against
conventional-segmented EPI and VFA-FLEET-Sinc at 3 T and 7 T. VFA-FLEET-SLR
showed reductions in both intermittent and stable ghosting compared to
conventional-segmented and VFA-FLEET-Sinc, resulting in improved image quality
with a minor trade-off in temporal SNR. Combining VFA-FLEET-SLR with
acceleration, we achieved a 0.6-mm isotropic acquisition at 7 T--without zoomed
imaging or partial Fourier--demonstrating reliable detection of BOLD responses
to a visual stimulus. To counteract the increased repetition time from
segmentation, simultaneous multi-slice VFA-FLEET-SLR was demonstrated using
RF-encoded controlled aliasing.
Conclusions
VFA-FLEET with a recursive RF pulse design supports acquisitions with low
levels of artifact and spatial blur, enabling fMRI at previously inaccessible
spatial resolutions with a "full-brain" field of view.Comment: 51 pages (including supplement), 8 main figures, 6 supporting
figures. For supporting videos (8), please visit
https://github.com/aveberman/vfa-fleet. Note: this work has been accepted for
publication at Magnetic Resonance in Medicin
Thermoresistant Newcastle disease vaccine effectivelyprotects SPF, native, and commercial chickens inchallenge with virulent virus
Background: Due to the more stability and a better homogenecity in immune response, the use of thermoresistant vaccines in different chicken types has been increased. Objective: This study aimed to evaluate the efficacy of a newly developed Newcastle disease vaccine (ND.TR.IR ) originating from 1-2 strain in specific pathogen-free (SPF) and native and broiler chickens. Methods: Following determination of pathogenicity indices on the candidate seed, three efficacy examinations were conducted. In the first experiment, 120 1-day-old SPF chickens were randomly allocated to six groups and either vaccinated with ND.TR.IR via eye drop at 1, 7, and 21 days of age (V-1, V-7, and V-21), or considered as non-vaccinated control groups (C-1, C-7, and C-21). At 20th post-vaccination day, sera hemag-glutination inhibition (HI) antibody titres against ND virus (NDV) were measured and then the chickens were challenged by virulent NDV (vNDV). In the second and third experiments, the efficacy of ND.TR.IR vaccine was compared to routine vaccination program (B1 and LaSota) in native and broiler chickens that were vaccinated at 10 and 20 days of age, respectively. The HI antibody titres were measured on 10, 20, 30, and 40 days of age, and also challenge efficacy test with vNDV was conducted on 30 days of age. Results: The studied virus, as a vaccinal seed, complied with the pathogenicity indices of avirulent NDV and molecular identity of 1-2 strain. In the efficacy evaluation trials, the vaccinated chickens had higher HI antibody titres against NDV compared with their corresponding control chickens (p < 0.05). Results of the challenge tests indicated 95% and 100% protection against vNDV in native, SPF, and broiler-vaccinated chickens, respectively. Conclusions: The present findings indicated that administration of ND.TR.IR induced appropriate HI antibody titres against NDV in SPF, native, and broiler chickens associated with good protection in efficacy test
Neural Correlate of Filtering of Irrelevant Information from Visual Working Memory
In a dynamic environment stimulus task relevancy could be altered through time and it is not always possible to dissociate relevant and irrelevant objects from the very first moment they come to our sight. In such conditions, subjects need to retain maximum possible information in their WM until it is clear which items should be eliminated from WM to free attention and memory resources. Here, we examined the neural basis of irrelevant information filtering from WM by recording human ERP during a visual change detection task in which the stimulus irrelevancy was revealed in a later stage of the task forcing the subjects to keep all of the information in WM until test object set was presented. Assessing subjects' behaviour we found that subjects' RT was highly correlated with the number of irrelevant objects and not the relevant one, pointing to the notion that filtering, and not selection, process was used to handle the distracting effect of irrelevant objects. In addition we found that frontal N150 and parietal N200 peak latencies increased systematically as the amount of irrelevancy load increased. Interestingly, the peak latency of parietal N200, and not frontal N150, better correlated with subjects' RT. The difference between frontal N150 and parietal N200 peak latencies varied with the amount of irrelevancy load suggesting that functional connectivity between modules underlying fronto-parietal potentials vary concomitant with the irrelevancy load. These findings suggest the existence of two neural modules, responsible for irrelevant objects elimination, whose activity latency and functional connectivity depend on the number of irrelevant object
Recommended from our members
Columnar Segregation of Magnocellular and Parvocellular Streams in Human Extrastriate Cortex
Magnocellular versus parvocellular (M-P) streams are fundamental to the organization of macaque visual cortex. Segregated, paired M-P streams extend from retina through LGN into V1. The M stream extends further into area V5/MT, and parts of V2. However, elsewhere in visual cortex, it remains unclear whether M-P-derived information (1) becomes intermixed or (2) remains segregated in M-P-dominated columns and neurons. Here we tested whether M-P streams exist in extrastriate cortical columns, in 8 human subjects (4 female). We acquired high-resolution fMRI at high field (7T), testing for M- and P-influenced columns within each of four cortical areas (V2, V3, V3A, and V4), based on known functional distinctions in M-P streams in macaque: (1) color versus luminance, (2) binocular disparity, (3) luminance contrast sensitivity, (4) peak spatial frequency, and (5) color/spatial interactions. Additional measurements of resting state activity (eyes closed) tested for segregated functional connections between these columns. We found M- and P-like functions and connections within and between segregated cortical columns in V2, V3, and (in most experiments) area V4. Area V3A was dominated by the M stream, without significant influence from the P stream. These results suggest that M-P streams exist, and extend through, specific columns in early/middle stages of human extrastriate cortex. SIGNIFICANCE STATEMENT The magnocellular and parvocellular (M-P) streams are fundamental components of primate visual cortical organization. These streams segregate both anatomical and functional properties in parallel, from retina through primary visual cortex. However, in most higher-order cortical sites, it is unknown whether such M-P streams exist and/or what form those streams would take. Moreover, it is unknown whether M-P streams exist in human cortex. Here, fMRI evidence measured at high field (7T) and high resolution revealed segregated M-P streams in four areas of human extrastriate cortex. These results suggest that M-P information is processed in segregated parallel channels throughout much of human visual cortex; the M-P streams are more than a convenient sorting property in earlier stages of the visual system
Recommended from our members
Smooth versus Textured Surfaces: Feature-Based Category Selectivity in Human Visual Cortex
Abstract In fMRI studies, human lateral occipital (LO) cortex is thought to respond selectively to images of objects, compared with nonobjects. However, it remains unresolved whether all objects evoke equivalent levels of activity in LO, and, if not, which image features produce stronger activation. Here, we used an unbiased parametric texture model to predict preferred versus nonpreferred stimuli in LO. Observation and psychophysical results showed that predicted preferred stimuli (both objects and nonobjects) had smooth (rather than textured) surfaces. These predictions were confirmed using fMRI, for objects and nonobjects. Similar preferences were also found in the fusiform face area (FFA). Consistent with this: (1) FFA and LO responded more strongly to nonfreckled (smooth) faces, compared with otherwise identical freckled (textured) faces; and (2) strong functional connections were found between LO and FFA. Thus, LO and FFA may be part of an information-processing stream distinguished by feature-based category selectivity (smooth > textured)
Thinking Outside the Box: Rectilinear Shapes Selectively Activate Scene-Selective Cortex
Fifteen years ago, an intriguing area was found in human visual cortex. This area (the parahippocampal place area [PPA]) was initially interpreted as responding selectively to images of places. However, subsequent studies reported that PPA also responds strongly to a much wider range of image categories, including inanimate objects, tools, spatial context, landmarks, objectively large objects, indoor scenes, and/or isolated buildings. Here, we hypothesized that PPA responds selectively to a lower-level stimulus property (rectilinear features), which are common to many of the above higher-order categories. Using a novel wavelet image filter, we first demonstrated that rectangular features are common in these diverse stimulus categories. Then we tested whether PPA is selectively activated by rectangular features in six independent fMRI experiments using progressively simplified stimuli, from complex real-world images, through 3D/2D computer-generated shapes, through simple line stimuli. We found that PPA was consistently activated by rectilinear features, compared with curved and nonrectangular features. This rectilinear preference was (1) comparable in amplitude and selectivity, relative to the preference for category (scenes vs faces), (2) independent of known biases for specific orientations and spatial frequency, and (3) not predictable from V1 activity. Two additional scene-responsive areas were sensitive to a subset of rectilinear features. Thus, rectilinear selectivity may serve as a crucial building block for category-selective responses in PPA and functionally related areas.National Institutes of Health (U.S.) (Grant R01 MH67529)National Institutes of Health (U.S.) (Grant R01 EY017081)Athinoula A. Martinos Center for Biomedical ImagingNational Center for Research Resources (U.S.)Mental Illness and Neuroscience Discovery (MIND) Institut
- âŠ